Approximately 3000 PGCs per sample were transferred into 1.5-mL tubes and resuspended in 60 L of wash buffer (20 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 0.5 mM spermidine, and protease inhibitors)
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The National Academy of Medicine recommends a total water intake (from all beverages and food) of approximately 125 ounces (3.7 liters) daily for men and 91 ounces (2.7 liters) for women under normal conditions
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ABSTRACT Recent advances in G protein-coupled receptor (GPCR) structural elucidation have strengthened previous hypotheses that multi-dimensional signal propagation mediated by these receptors is, in part, dependent on their conformational mobility