The inflammatory marker C-reactive protein (CRP) contributes to leptin resistance and weight gain by binding to leptin and reducing its signaling ability (Chen 2006
ConradCLympJThompsonVDunnCDaviesZChatfieldBet al
The line between "take Miralax" and "call the doctor" is usually whether symptoms are progressive, severe, or accompanied by red flags like vomiting or inability to pass gas
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The primary antibodies used included anti-ZO-1 (Abcam, ab307799, 1:5000), anti-Occludin (Abcam, ab216327, 1:5000), anti-ACSL4 (Abcam, ab155282, 1:10000), anti-TFR1 (ABclonal, A5865, 1:2000), anti-SLC7 A11 (Abcam, ab175186, 1:5000), anti-GPX4 (Abcam, ab125066, 1:5000), anti-VDAC2 (Proteintech, 116631-AP, 1:5000), anti-VDAC3 (Proteintech, 552601-AP, 1:5000), -tubulin (Proteintech, 100681-AP, 1:5000), and GAPDH (Proteintech, 104941-AP, 1:20000)