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The assay is based on reversed-phase high performance liquid chromatography with UV detection and simultaneous reduction of disulfide bound with tris(2-carboxyethyl)phosphine and the selective pre-column derivatization of the thiol group with 1-benzyl-2-chloropyridinium bromide

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Bovenschen et al 2 reported a woman developing ACD 24 to 48 hours after applying three DHA-containing sunless tanners

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Meet the Experts : David Merrill, M.D., Ph.D., geriatric psychiatrist and director of the Pacific Neuroscience Institutes Pacific Brain Health Center at Providence Saint Johns Health Center in Santa Monica, CA