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H., & Zhang, Z
The assay was carried out in the presence of mouse retinal lysate containing an enzyme buffer mixture (50 mM Tris-HCl [pH 7.4], 100 mM KCl, 5 mM MgCl 2 , 1 mM ADP, 0.5 mM PEP, and 0.2 mM NADH [reduced form of NAD + ]) and 8 U of LDH with a reaction volume of 1.0 ml
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Massons trichrome staining was performed on the sections to observe collagen deposition
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