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Wiley Online Library, John Wiley & Sons
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The cells were subsequently treated with DCFH-DA (10 M, Bio-lab) for 1 h in the dark, and washed three times with PBS
However, variant interpretation is complicated by factors such as membrane targeting: for instance, a serine mutation at one of the other Na + -binding residues, N210, causes a reduction in uptake, but also shows mixed localisation, 11 which may itself contribute to disease
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