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and rhombencephalon (the hindbrain)

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Marquez-Meneses JD, Olaya-Bonilla SA, Barrera-Carreo S, Tibaduiza-Arvalo LC, Forero-Crdenas S, Carrillo-Vaca L, Rojas-Rodrguez LC, Calderon-Ospina CA, Rodrguez-Quintana J

The cells were fixed with 4% formaldehyde and incubated with permeabilization buffer (0.3% Triton X-100 and 1% Goat serum in PBS) containing primary antibodies against dsRNA J2 ((1:1000) Scicons 10010500) and PABPC1 ((1:100) Abcam ab21060) followed by incubation with DAPI (1:1000) and conjugated secondary antibodies anti-mouse Alexa555 and anti-rabbit Alexa647 (1:500, Thermo Fisher Scientific)

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