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In summary, using a 96-well microplate, 20 L of serum samples were added to separate wells containing the Master Reaction Mix

Hydration Sufficient intake of water helps metabolize, and hence avoids dehydration while exercising

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Samples were resuspended in 2% acetonitrile (ACN), 0.05%TFA and peptide concentrations were estimated with 205 nm absorbance readings (Nanodrop, Thermo Fischer)

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