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Subsequent studies demonstrated that apelin signaling is highly context dependent, with differential coupling to G_i, G_q, and -arrestin pathways depending on ligand structure, receptor density, and tissue environment (Murali and Aradhyam, 2023)

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In our initial phenotype-driven screen, 238 semi-cloned embryos were produced from Trim-library-1 and Trim-library-2, among which two embryos exhibited PGC failure, and two others showed reduced PGC numbers (Supplementary information, Fig