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To analyze the effect of GSH on the activity of the pFeSAN nanozyme, 20 L of pFeSAN or GSH-pretreated pFeSAN (1 mg/mL), 20 L of TMB (50 mM) and 960 L of HAc-NaAc buffer (pH 4.0) were mixed and incubated at 25C for 10 min
Pharmacokinetic analysis showed a significant reduction in both the maximum serum concentration (Cmax) and area under the curve (AUC) for acetaldehyde in the GSH group, further confirming the efficacy of GSH in accelerating acetaldehyde clearance from the bloodstream
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These findings should be positioned as hypothesis-generating, and their clinical translatability requires validation in larger samples with pre-specified endpoints (113116)
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