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For WKY and SHR rats, they were given a wash-out period of 3 days before they received a different dose (i.e

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Briefly, an XhoI/EcoRI fragment containing a U6-shRNA cassette was amplified from pLKO.1 vector using high-fidelity PCR and ligated into XhoI/EcoRI-digested adenoviral shuttle vector pacAd5 K-NpA

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