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Vitamin B12 is a water-soluble vitamin, also known as cobalamin
Concentration = Total Peptide (mg)BAC Water (mL) = 10 mg2.5 mL = 4 mg/mL Calculate your draw volume
For separate detection of the two forms, a portion of the sample was treated with 5% 2-vinylpyridine (Sigma) for 60 min to trap GSH
The reasons for the detection of bands 1 and 2 in the oxidized state will be further discussed in the Discussion section
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