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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment
Peptide Vials Math assumes 10 mg vials reconstituted with 2 mL BAC water (concentration 5,000 mcg/mL)
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Regardless of the specific product, GLP-1 pens should never be exposed to temperatures above their labeled maximum (either 86F/30C or 77F/25C, depending on the product)
Published and discussed effects include modulation of the stress response, with cortisol levels and cortisol modulation the usual measures, and framings around stress resilience and stress adaptation
