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This alteration is accompanied by a metabolic change from glycolysis to a more oxidative form given by a decrease in phosphoglycerate mutase 1 (PGAM1), ALDOA, LDHA/B, and an increase in succinate dehydrogenase (SDH) A/B/C/D from the ADP-forming beta subunit of succinate-CoA ligase (SUCLA2), aconitase 2 (ACO2), PDK, and pyruvate dehydrogenase phosphatase (PDP), among others
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Sample sizes were guided by prior experience with the db/db mouse model and were designed to provide 80% power ( = 0.05) to detect biologically meaningful differences in metabolic outcomes
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