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Results were presented as pictogram or nanogram of mediator released per milliliter of conditioned medium

According to the manufacturers protocol, we used 1 g of RNA for complementary DNA (cDNA) synthesis with an Invitrogen cDNA reverse transcription kit

injection volume: 3 L

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In diabetic neuropathy, chronic hyperglycemia leads to peripheral nerve injury, altered nerve Fiber Na + , K + -ATPase activity, reduced nerve conduction velocity and the release of ROS which leads to the activation of NLRP3 inflammasome (Niulescu et al