Key Takeaways 4 peptides Perfect B uses clinically for hair growth: GHK-Cu (copper peptide), PDRN (salmon DNA), CJC-1295/Ipamorelin (GH pathway), and the BPC-157/TB-500 Wolverine stack
Cell culture and transfection WT HepG2 or stably transfected cells (pSuperior.puro-GSTM2) were cultured in DMEM (HyClone, Logan, UT, USA) containing 10% foetal bovine serum (Clark Bioscience, Richmond, VA, USA), 100 U/mL penicillin, and 100 g/mL streptomycin in dishes at 37 C and transfected with Lipo6000 Transfection Reagent (#C0528, Beyotime)
The peptide mixture was loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 m 2 cm, nano Viper C18) connected to the C18-reversed phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 m inner diameter, 3 m resin) in buffer A (0.1%formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology
R.DuphorneC
KPCW Co-Host / Producer of The Mountain Life and Cool Science Radio Pete Stoughton is the Brand Relations Manager at Trail and Ultra Running as well as the Director of Programs at CCPC