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To identify glutathione-overproducing mutants, acrolein was used as a selective agent, given glutathiones key role in protecting cells from acrolein-induced toxicity (Mano et al., 2009

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Membranes were then subjected to BSA (2%4%) for 1 h and then incubated with the appropriate antibodies and subsequently with appropriate secondary antibodies conjugated to horseradish peroxidase

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