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To measure PIP 3 , FoB were stimulated with 5 g/mL anti-IgM (Jackson Immunoresearch 715-006-020) for the indicated time points, then fixed in 2% FA and permeabilized with 0.1% saponin
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The authors acknowledge the Monash Proteomics and Metabolomics Facility (Parkville Node) for providing LC-MS technical assistance
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