Methods Construct modification and expression of full-length GLP-1R The optimized gene of full-length GLP-1R was cloned into the modified pFastbac1 vector at the BamHI/HindIII sites and the native signal sequence was replaced by haemagglutinin (HA) to enhance receptor expression (see primers in Supplementary Table 2), followed by a flag-tag, a 10 His-tag and a tobacco etch virus protease (TEV) site at the N-terminus
The distance between the plasma membranes of the MCs and pre-terminal axons was less than 100 nm and in some points they seemed to be in contact 25
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Cao Y, Cheng K, Yang M, Deng Z, Ma Y, Yan X, et al