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Retention behavior helps here, since the highly charged fragment, the small polar complex and the mid-length peptide are unlikely to co-elute, but resolution is a prerequisite for quantitation, not a substitute for it

Further studies revealed that treatment with N-acetylcysteine (NAC), a GSH precursor, significantly upregulates the GSH/GSSG ratio and increases the expression of GPX4, NRF2, and HO-1, effectively inhibiting ferroptosis (Zhang S

Beinart N, Hackett RA, Graham CD, Weinman J, Ostermann M (2015) Mood and illness experiences of adults with cystinosis

As a tripeptide, glutathione is subject to destruction in the small intestine [35]