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Coimmunoprecipitation (co-IP) assay and immunoblotting Striatum tissues and cells were lysed using RIPA lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, and 0.5% NP-40) containing protease and phosphatase inhibitors for 1 h on ice and sonicated 3 times using a Sonics VC750 ultrasonic processor

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Chronic conditions such as allergic asthma, allergic rhinitis, food allergy, atopic dermatitis, and anaphylaxis impair quality of life and contribute to substantial healthcare expenditures (143)

Metabolic health interest: Some clinicians consider it when visceral fat and insulin resistance patterns are part of the picture