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Previously it has been shown that enhanced HR-type necrotization in TMV-infected NahG tobacco is indeed correlated to oxidative stress, as marked by the accumulation of ROS ( 1 hybrids that express nahG (CEMK-9 X NahG and TRI-2 X NahG F 1 ), wild type levels of TMV resistance (including HR necrosis) are coupled to hyperaccumulation of GSSG, an indication of constant suppression of oxidative stress in virus-infected plant cells

Directly supplementing with oral reduced glutathione (L-GSH), however, presents a fundamental pharmacokinetic challenge: GSH has a plasma half-life of approximately 1.6 minutes, undergoes rapid hydrolysis in the gut by intestinal -glutamyl transpeptidase (-GT), and cannot cross cell membranes intact requiring full breakdown into constituent amino acids and intracellular resynthesis

At 48 h post-injection, the mice were euthanized to collect tumors and major organs for subsequent ex vivo imaging

Kumar P, Liu C, Suliburk J, Hsu JW, Muthupillai R, Jahoor F, et al

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