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The recycling reaction was initiated by the addition of reaction buffer (100 mM sodium phosphate, pH 7.4), supplemented with 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) (100 M) (Sigma), NADPH (100 M) (Roth), glutathione reductase (1 U/ml) (Sigma), and EDTA (1 mM) (Sigma)
Jorge De Vicente
Heres the vitamin D I recommend
The resulting GSH or homoglutathione (-L-glutamyl-L-cysteinyl--alanine) conjugates were usually much less toxic and more water-soluble than the original xenobiotics (Brown and Neighbors, 1) (Bartling et al., 2002
Amino acids L-Alanine (AAA1580414), L-Cystine (AAA1376218), L-Glutamine (AAA1420118), L-Histidine (AAA1762718), L-Methionine (AAA1031822), L-Phenylalanine (AAA1323814), L-Proline (AAA1019914), L-Serine (AC132660250) and L-Tyrosine (AAA1114118) were obtained from Thermo Fisher
