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It activates Chk1, which downregulates MAD2 and prevents its kinetochore localization, thereby allowing the APC/C to trigger premature anaphase

PubMed Link Zamani B, et al

In one melanoma study, mice exposed to higher NAC doses experienced greater metastatic activity than those given lower doses, suggesting the effect may be dose-dependent in certain experimental settings.42 Researchers have not established whether these findings apply to humans, but starting with a moderate dose offers a more conservative approach while still aligning with a large portion of the clinical literature

DOI: 10.1056/NEJMoa2107519

It is also used to make the important brain chemical acetylcholine