Wang Y, Kuang S, Li K, Chen S, Yang M, Deng K, et al
For de novo NADPH synthesis pathway, correlation studies have revealed that thionicotinamide adenine dinucleotide (NADS) and thionicotinamide adenine dinucleotide phosphate (NADPS), converted from the pro-drug thionicotinamide (TN), act as inhibitors of NADKs through targeting the NAD-binding site of NADKs and decreasing the levels of NADPH
siRNA transfections were performed using Lipofectamine 2000 and ON-TARGETplus Human SMARTpool siRNAs (Dharmacon) against TYMS and a scrambled control pool, according to the manufacturers instructions
Cyanocobalamin is contraindicated in these individuals as the cyanide moiety may worsen optic nerve damage
Eur J Endocrinol (2003) 148(4):38993