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Periodic blood work helps monitor ongoing response and safety
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Despite a relatively short half-life of approximately 30 minutes, the peptide triggers gene expression changes that persist and cascade independently of its continued presence
Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma #IPVH00010), blocked for an hour using 5% milk in TBST, and stained overnight with targeting primary antibodies in 5% milk TBST
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