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Briefly, ring-stage cultures were tightly synchronised as described for ring stage survival assays and treated with a defined quantity of SLO units for 10-20 min at 37 C to preferentially lyse uninfected erythrocytes

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Relative gene expression was calculated after normalization to a house-keeping gene (mouse or human 18S rRNA)

In total, this review highlights exerkines as candidate mediators and biomarkers of exercise-retina crosstalk and outlines priorities for mechanistic validation and clinical translation alongside established therapies such as anti-VEGF treatment

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