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Uptake of CLF To measure CLF polarity, organoids were removed from the Matrigel by washing with PBS and incubated with culture medium supplemented with 10 g/ml CLF (Corning Inc.) and 1 g/ml Hoechst 33342 for 30 minutes at 37 C in a 5% CO 2 incubator

Mechanisms of Semaglutide-Induced Gastrointestinal Effects: Chen, M., Patel, K., & Gomez, R