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Plates were subsequently washed with 0.1% polyethylene sorbitan monolaurate (Tween20)-Tris-buffered saline (TTBS), immediately fixed with cold 3.7% paraformaldehyde in PBS, and re-treated with the blocking solution overnight at 4C

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It uses glutathione reductase and NADPH to amplify a colorimetric signal that standard spectrophotometers can detect with high reproducibility

Interestingly, our supplement had an impact to increase testicular glutathione level, expression of the Nrf2 gene and decrease MDA content