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The copper(II) ion is coordinated by the glycine amino terminus, the deprotonated glycine to histidine peptide-bond nitrogen, and the histidine imidazole nitrogen, giving the complex its characteristic colour and stability
When sequential coupling is used, the synthesis yield of the crude peptide is 83%, the purity of the crude peptide is 65.3%, the purification yield is 23.6% and the purity of the refined peptide is 99.02%
Daley S et al
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