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Stability studies were performed to check the quality of drugs under various stress conditions like acidic, basic, peroxide, thermal and photolytic

prepare aliquots if needed and avoid freezethaw

The CYP27B1 gene is located on chromosome 12q14.1 and is composed of 9 exons that encode a 508 amino acid protein

Quantitative real-time polymerase chain reaction (qRT-PCR) In accordance with previous studies [36], we identified 10 mRNAs for real-time fluorescent quantitative PCR (qRT-PCR) verification (Additional file 1)