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In addition, Fe 2+ is a cofactor of lipoxygenases (LOXs) that catalyzes PUFA-containing phospholipids into pro-ferroptotic lipid peroxidation (Yang et al., 2016)
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Approximately 3000 PGCs per sample were transferred into 1.5-mL tubes and resuspended in 60 L of wash buffer (20 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 0.5 mM spermidine, and protease inhibitors)
They can range from mild to moderate and may include cramping, bloating, or a rash, in case of overdose