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10.1016/j.bbrc.2015.10.107 40
Features of Glutathione Agarose: Slurry crosslinked 6% beaded agarose, slurried in water with sodium azide High capacity binds at least 40 mg of purified recombinant GST protein per milliliter of resin High yield and purity consistently purifies at least 10 mg of GST-tagged protein per milliliter of resin with greater than 90% purity Cost-effective resin is economically priced and can be reused at least five times without reduction in binding capacity and purification performance Versatile works well to purify GST-fusion proteins from bacterial lysates or use with pre-purified GST-tagged proteins to pull down protein interactions Compatible validated and effective for use with Thermo Scientific Cell Lysis Reagents to extract and purify from bacterial or mammalian cell cultures The high-quality support consists of glutathione that has been immobilized by its central sulfhydryl group via a 12-atom spacer arm to crosslinked 6% beaded agarose
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