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The EB dye content was calculated using the following formula: Measurement of mean arterial pressure (MAP) Systolic and diastolic blood pressure was recorded by the tail-cuff technique using an NIBP ML125 system (ADInstruments, Australia.blood pressure was measured by placing animals tail in the cuff of a monometer

Furthermore, the study noted an apparent reduction in myeloperoxidase (MPO) activity, which is primarily found in neutrophils and is an enzyme involved in the model's response to inflammation