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The infected mice exhibited a higher increase in interleukin (IL) IL-5, IL-4, and IL-2 than IL-12 and interferon- (IFN-), suggesting an important alteration in cytokine profile from Th1 to Th2 (Figure 2) [38]

Western blot analysis and Coomassie Brilliant Blue (CBB) staining Samples were boiled in sample loading buffer at 100 C for 10 min, separated by SDS-PAGE, and transferred onto NC membranes for blotting

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Oxidative stress can contribute to fatigue and low energy levels