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Chanson P, Salenave S
The extract was rotary evaporated at 30 C to a volume of 2 ml, acidified to pH 4 with formic acid, and 200 l aliquots were purified using preparative HPLC (Agilent Technologies, 1260 Infinity) over a Luna 5 m phenyl-hexyl 100 (250 10 mm) column and a 15 min linear gradient from 0% acetonitrile in H 2 O to 16% acetonitrile in H 2 O at 4 ml min 1 flow
K., McCartney, C
Building a MOTS-C Protocol Within a Longevity Program A MOTS-c protocol is most powerful not as a standalone intervention but as one layer of a structured longevity program that addresses mitochondrial health, metabolic flexibility, and body composition simultaneously
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