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GST-Pns11 or GST-Pelo was incubated with glutathione-Sepharose beads (Amersham, 17-0756-01) for 5 h at 4 C, centrifuged for 5 min at 500 g , and then the supernatant was discarded
And then Ill place a small revision, OK
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Clean the vial stopper and injection site with alcohol swabs
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