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All these changes lead to cytokine storm characterized by increased expression and release of proinflammatory cytokines that participate in the pathogenesis of ARDS during virus respiratory infections like COVID-19

They measured the apparent levels of messenger RNA (mRNA) associated with urea cycle enzymes in the liver, evaluated the overall nitrogen balance, and hypothesized the nitrogen contents of various organs

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After incubating at 37 C for 2 h, the samples were centrifuged at 360 g for 10 min, and then each sample (200 L) was transferred to a 96-well plate