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Next, 500 L B-PER II (product 78260, Thermo Fisher Scientific, Waltham, MA) containing 100 g/mL lysozyme (MilliporeSigma L6876) and 5 U/mL DNase I (Thermo Fisher 90083) was added to cell pellets for harvesting, and all samples were vortexed and incubated at 37 C for an additional 15 min

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Some of the biggest risk factors for B12 deficiency are vegetarian/vegan diets, pregnancy/breastfeeding, and aging

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