Preparation of NEM-derivatized cysteine and GSH internal standards The N-ethylmaleimide (NEM) derivatized isotope labeled [ 13 C 3 , 15 N]-cysteine-NEM and [ 13 C 2 , 15 N]-GSH-NEM were prepared by derivatizing the [ 13 C 3 , 15N]-cysteine and [ 13 C 2 , 15 N]-GSH standards with 50 mM NEM in 10 mM ammonium formate (pH = 7.0) at room temperature (30 min) as previously described 95
However, individual results can vary based on factors like starting weight, dosage, and lifestyle habits
The behavioral test was proceeded 4 weeks after gavage
Chen C, Zheng H, Xu J, Shi X, Li F, Wang X
Chronic diseases including diabetes, HIV, and hepatic cirrhosis impair antioxidant capacity