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The glycerol content in the incubation medium served as an indicator of lipolysis and was measured via the GPO-Trinder reagent (Sigma, FG0100) according to the manufacturers instructions

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doi:10.1002/oby.23556

Days 2 and 3: side effects peak (if they come at all) If you are going to experience gastrointestinal side effects from your first dose, days two and three are when they most commonly appear

J Med Chem (2014) 57(8):320512.10.1021/jm401992e 107 KumarKVGuptaAK