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Restore Sleep, Metabolic and Gut Function Sleep apnea, insomnia, glucose dysregulation, pain, alcohol, medication burden and intestinal inflammation can perpetuate symptoms even when hormones are corrected

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LCMS/MS analysis was performed using an Advance nanoLC (BrukerMichrom, Auburn, CA, USA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific) equipped with a NANOHPLC capillary column C18 (0.075 mm ID 150 mm length, 3 m particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 535% CH 3 CN /0.1% formic acid) at a flow rate of 300 nL/min

NIH R01HL158555