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Pereira-Nunes A, Simes-Sousa S, Pinheiro C, Miranda-Gonalves V, Granja S, Baltazar F

Dual digestion ensured overlapping fragments and full sequence coverage, including modified Lys20

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Loading GST-fusion proteins onto glutathione beads steps: Now that you have your protein of interest bound to the glutathione agarose beads through GST, youll want to add the potential interacting proteins to this mixture