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The fully differentiated adipocytes were treated with 20 nmol/l exendin-4 (E7144, Sigma-Aldrich, St Louis, MO, USA), 10 mol/l SRT1720 (S1129, Selleck, Houston, TX, USA), 30 mmol/l nicotinamide (N0636, Sigma-Aldrich, USA), a combination of exendin-4 and SRT1720, or a combination of exendin-4 and nicotinamide, for 24 h, respectively
minimal weight loss expected
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