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Active Akt phosphorylates multiple targets including Bad (preventing it from inhibiting anti-apoptotic Bcl-2), MDM2 (stabilizing p53), FOXO transcription factors (preventing pro-apoptotic gene expression), mTOR (promoting protein synthesis)

Using cell culture models and molecular analysis techniques including cDNA microarray, RT/real-time PCR, and Western blotting, researchers demonstrated that BPC-157 treatment upregulates GHR expression in a dose- and time-dependent manner at both the mRNA and protein levels