Following three 5-min washes with TBST, sections were incubated for 2 h at room temperature with fluorophore-conjugated secondary antibodies: goat anti-rat IgG Alexa Fluor 488 (Invitrogen) and goat anti-rabbit IgG Alexa Fluor 532 (Invitrogen)
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This research received funding from the Changzhou Sci-Tech Program Applied Basic Research General Project (CJ20245013, Tongbin Chen), Changzhou Medical Center of Nanjing Medical University Basic Research Program (CMCB202417, Xi Chen) and Top Talent of Changzhou The 14th Five-Year Plan High-Level Health Talents Training Project (2024CZBJ011, Xi Chen) and 2022 Annual Youth Talent Science Project of the Health Commission of Changzhou City (QN202217, Wenyue Da)
Adequate protein and micronutrient intake support hormone production