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Both RNS and ROS can damage contractile proteins, enzymes, and mitochondria through oxidative and nitrosative processes, intensifying stress signaling, exacerbating cellular dysfunction, and ultimately leading to myocardial and endothelial cell (EC) death [56]

Additionally, the CheckMate 18 phase III clinical trial, focusing on individuals with inoperable or metastatic melanoma, revealed a 43.6% objective response rate (ORR) in PD-L1-positive patients treated with nivolumab, compared to 9.1% in those receiving chemotherapy [116]

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Blanco) were derived from E14.5 d embryos, SV40 T-antigen transformed, and grown in Dulbeccos modified Eagles medium (DMEM, Corning) containing 10% fetal bovine serum (VWR Life Sciences, Seradigm) and 100 g/mL penicillin (Sigma) at 37 C and 5% CO 2