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The elution profile of the sample homogenized in BSAN buffer (blue tracing) is characterized by the presence of both the two symmetrical peaks corresponding to the GS-NEM conjugate (4.29 min and 4.95 min r.t.) and the peak corresponding to the excess of NEM (5.8 min r.t.)
Genetic factors, baseline metabolic health, dietary compliance, physical activity levels, and even gut microbiome composition likely contribute to this variability
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