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This vital mineral serves important functions in the body, such as supporting hemoglobin production, promoting muscle function, and maintaining healthy connective tissues.*

doi: 10.1016/j.molmet.2020.101102 37 ManigaultKRThurstonMM

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Soluble His-tagged proteins were purified with NiNTA (Qiagen) as previously 56 , and the eluted proteins were dialyzed against buffer H (20 mM TrisHCl, pH 8.0, 100 mM NaCl, 10% glycerol, and 1 mM MgCl 2 )