JAMA 310 , 24352442 (2013)
More experimental studies using cell and animal models of immune-related disorders are necessary to further understand the underlying mechanisms, together with elaborate preclinical pharmacokinetics, pharmacodynamics, bioavailability, and toxicity studies
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Briefly, cell pellets or tissues were homogenized in 1 ml TRIzol Reagent (Ambion, Life Technologies, CA), total RNAs were separated from other cell contents by sequential chloroform and isopropanol precipitation
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