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The samples were resolved on an ACQUITY UPLC BEH C18 column (100 2.1 mm i.d., 1.7 m particle) using water and acetonitrile as mobile phases A and B, respectively, at a flow rate of 0.15 ml min 1 and a column temperature of 40 C

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Interaction with Cav-1, however, avoids excessive or aberrant eNOS activity, and Cav-1 deficiency can cause pulmonary hypertension and cardiomyopathy (Drab et al., 2001